引用

临床上，胆道梗阻常伴有进行性炎症。脱氢穿心莲内酯(DA)具有抗炎作用。然而，DA在胆汁淤积性肝损伤中的抗炎作用尚不清楚。小鼠第1天胆管结扎(BDL)后腹腔注射DA。然后小鼠经回盲静脉注射脂多糖(LPS)。在LPS作用的BDL小鼠中评估肝功能标志物、组织学、促炎细胞因子水平、NF-B激活和纤维化形成。用高含量细胞仪检测LPS与原代Kupffer细胞的结合。DA可显著降低LPS诱导的BDL小鼠血清和肝脏中丙氨酸转氨酶(ALT)和总胆红素(TBIL)的水平。DA具有肝脏保护作用，这也被LPS延长BDL小鼠的生存时间所证实。肝脏组织病理学显示，DA治疗后炎性细胞浸润、胆管增生和胆道坏死减少。 Furthermore, DA reduced the expression levels of tumor necrosis factor (TNF)- and interleukin (IL)-6 in liver tissue and plasma and showed decreased NF-B activation in BDL mice with LPS. DA could prevent LPS binding to primary Kupffer cells in the normal liver and BDL mice liver. DA also suppressed LPS-stimulated inflammatory responses by blocking the interaction between LPS and TLR4 in primary Kupffer cells and human LX-2 cells, thereby inhibiting NF-B activation. DA inhibition of inflammation against liver damage following BDL with LPS may be a promising agent for the treatment of cholestatic liver injury. 2018 The Author(s). Published by S. Karger AG, Basel.