引用

电针能有效缓解带状疱疹后神经痛(PHN)患者的疼痛。然而，EA治疗PHN的机制尚不清楚。在成年大鼠全身注射TRPV1激动剂的超效类似物树脂亚毒素(RTX)可以通过消融表达TRPV1的感觉神经元来重现PHN的临床症状。在本研究中，我们确定了EA对PHN大鼠模型的有益作用及其潜在机制。，单次注射RTX诱导大鼠PHN。用辐射热刺激测试热痛觉过敏，用冯弗雷细丝量化机械异常痛。免疫荧光标记显示TRPV1受体。采用电镜观察坐骨神经的超微结构变化。利用经神经节示踪剂霍乱毒素b亚单位(CTB)绘制脊髓背角有髓鞘初级传入末梢的萌芽。RTX注射可降低热敏感性，并在3周内逐渐诱导触觉异常痛。以2和15 Hz电刺激GB30和GB34，而不是100 Hz电刺激，可显著提高治疗后4周的热敏感性，并降低治疗后2周的触觉异常痛。在2和15 Hz的电刺激下，rtx处理的大鼠脊髓浅背角中trpv1阳性背根神经节神经元及其传入纤维中央末端的损失得以恢复。 Moreover, EA significantly reduced the loss of unmyelinated fibers and the damage of the myelinated nerve fibers of RTX-treated rats. Furthermore, EA at 2 and 15 Hz inhibited the sprouting of myelinated primary afferent terminals into the spinal lamina II of RTX-treated rats.,EA treatment improves thermal perception by recovering TRPV1-positive sensory neurons and nerve terminals damaged by RTX. EA Also reduces RTX-induced tactile allodynia by attenuating the damage of myelinated afferent nerves and their abnormal sprouting into the spinal lamina II. Our study provides new information about the mechanisms of the therapeutic actions of EA in the treatment of PHN.