引用

昼夜节律系统(CTS)是由一组负责产生和调节昼夜节律的神经结构组成的。下丘脑的视交叉上核(SCN)是产生昼夜节律性的结构，24小时环境光暗循环(LD)是它的主要同步器。SCN在各种间脑、端脑和前脑神经结构中产生昼夜节律的表达。最近，“扩展STC”的概念被提出，考虑到大约有100个神经结构可以组成这个系统。在这一背景下涉及的几个区域中，脑室旁下区(SPVZ)从SCN接收大量输入，已经被强调，尽管尚未在灵长类物种如卡利思克斯jacchus进行研究。我们的研究目的是描述其细胞结构和神经化学成分，除了绘制狨猴的视网膜传入到SPVZ。为此，绒猴大脑的额部切割用nisl法和免疫组化技术进行染色。从我们的结果中，我们可以通过nisl方法和神经元特异性核(NeuN)和胶质纤维酸(GFAP)蛋白的免疫反应性来表征该物种的SPVZ细胞结构。我们发现SPVZ位于SCN的背侧和尾侧之间，以及下丘脑室旁核(PVN)的腹侧，向外侧分布到III脑室，占据心室周围部分。钙结合蛋白的神经化学含量表明，钙结合蛋白(CB +)的免疫反应神经元比钙结合蛋白(CR)的免疫反应神经元和小白蛋白(PV)的免疫反应神经元更明显。 The CB + neurons present a dorsolateral, and occupy the ventromedial, ventrolateral and perisuprachiasmatic ventrolateral regions of the SPVZ. It was also possible to observe in the ventromedial portion of SPVZ immunoreactive neural fibers to neuropeptide Y (NPY), decarboxylase of glutamic acid (GAD) enzyme, and substance P (SP), whereas in the ventral and dorsal portions of SPVZ we observed 5-HT immunoreactive fibers. The glutamate receptor subunit 1 (GluR1) is strongly present in the dorsal SPVZ. We also observed a bilateral distribution of immunoreactive retinohypothalamic tract axon terminals (RHT) to the b subunit of cholera toxin (CTb), which demonstrates the projection of the retina to the ventral and ventrolateral perisuprachiasmatic portion. Although we did not perform functional experiments, the clear presence of CB in the SPVZ, its intimate relation with the SCN and PVN, together with the data available in the literature, places the SPVZ in marmosets as an important region of the extended CTS.