引用

神经损伤后骨骼肌会出现不同程度的萎缩和代谢障碍。神经营养因子是肌肉再生所必需的。人羊水来源干细胞(AFS)具有分泌神经再生所需的各种神经营养因子的潜力。在本研究中，我们在肌肉去神经和神经吻合模型中，通过肌内注射AFS来评估神经功能的结果。实验选取体重200 ~ 250 gm的sd大鼠72只。肌肉去神经模型为坐骨神经横切，近端缝合入臀肌。神经吻合模型为坐骨神经横向切除后再接4针。这些动物被分为三组:对照组、电肌刺激组和AFS组。、NT-3(神经营养因子3)、BDNF(脑源性神经营养因子)、CNTF(睫状神经营养因子)、GDNF(胶质细胞系源性神经营养因子)在AFS细胞和培养液上清中均有高表达。肌内注射AFS可使神经远端和失神经肌的多种神经营养因子显著表达。AFS引起失神经肌中Bcl-2的高表达，Bad和Bax的相互降低。 AFS preserved the muscle morphology with high expression of desmin and acetylcholine receptors. Up to two months, AFS produced significant improvement in electrophysiological study and neurological functions such as SFI (sciatic nerve function index) and Catwalk gait analysis. There was also significant preservation of the number of anterior horn cells and increased nerve myelination as well as muscle morphology.,Intramuscular injection of AFS can protect muscle apoptosis and likely does so through the secretion of various neurotrophic factors. This protection furthermore improves the nerve regeneration in a long term nerve anastomosis model.