引用

本研究的目的是比较人胶质限制性祖细胞(hGRPs)促进不同束轴突生长的功效。我们检查了移植到颈椎(C4)背柱病变以测试感觉轴突的hGRPs，以及移植到C4半球以测试运动束的hGRPs的潜力。从冷冻标本中解冻的hGRPs悬浮在PureCol基质中，并急性移植到C4背柱或半切病变中。对照组大鼠只服用PureCol。移植5周后，所有移植细胞在背柱病变的大鼠中存活，但只有大约一半的移植细胞在半切区存活。在背柱病变组，很少有感觉轴突向对照组病变部位短距离生长。移植后hGRPs的存在显著促进了轴突的生长。在半切组，蓝脊髓轴突在对照组和移植组的延伸相似，hGRPs的存在没有增强。即使在hGRPs存在的情况下，rubbrospinal轴突也没有生长到病变中。然而，网状脊髓轴突和raphspine轴突在移植体内生长的距离显著延长。 These results demonstrate the differential capacity of axonal growth/regeneration of the motor and sensory tracts based on their intrinsic abilities as well as their response to the modified environment induced by the hGRPs transplants. We conclude that hGRP transplants can modify the injury site for axon growth of sensory and some motor tracts, and suggest they could be combined with other interventions to restore connectivity. Copyright 2018. Published by Elsevier B.V.