引用

外围的感觉神经元轴突损伤强烈提高脊髓中央轴突的再生。目前尚不清楚什么分子发起这样的调节效应。因为ATP释放细胞外地由神经和其他组织损伤,我们假设注入ATP为周围神经可能模仿神经损伤的刺激效应在初级感觉神经元的再生状态。我们发现一个注射6 l 150 ATP的雌性大鼠坐骨神经轴突的数量翻了两番后成长为一个在脊髓损伤中心伴随脊柱横断。第二个促进ATP注射一周后第一个单一注射的刺激效果明显增强。单一ATP注入phospho-STAT3和GAP43的表达增加,再生活动的两个标记,在感觉神经元。双ATP注射持续phospho-STAT3和GAP43的激活,这可能占整个病灶中心标记轴突生长。进行类似的研究或P2Y2 P2X7受体基因敲除小鼠显示P2Y2受体参与STAT3的激活ATP注入或调节损伤后,P2X7受体则不可见。注射引起的ATP,海拔150米的小沃勒变性和行为测试显示没有明显的长期不良对坐骨神经功能的影响。在这个研究结果揭示可能的机制刺激再生项目,建议实际策略促进轴突再生脊髓损伤后。意义STATEMENTInjury外围的感觉神经元轴突的被强烈提高脊髓中央轴突的再生。 In this study, we found that injection of ATP into a peripheral nerve can mimic the effect of peripheral nerve injury and significantly increase the number of sensory axons growing across lesion epicenter in the spinal cord. ATP injection increased expression of several markers for regenerative activity in sensory neurons, including phospho-STAT3 and GAP43. ATP injection did not cause significant long-term adverse effects on the functions of the injected nerve. These results may lead to clinically applicable strategies for enhancing neuronal responses that support regeneration of injured axons.