引用

我们最近表明,外源性和内源性神经肽Y (NPY)降低交感神经活动和压力反射功能通过一个行动Y1受体(Y1R)下丘脑室旁核(PVN)。然而,是否NPY直接作用于presympathetic神经元投射到延髓腹外侧的髓质(RVLM)是未知的。为了验证这个假设,我们用免疫荧光来确定Y1R出现在PVN神经元RVLM项目,之前被RVLM显微镜下注射的逆行示踪霍乱毒素B亚基(施,0.25%;实验室列表)。NPY Y1R-immunoreactivity (ir) (1:10 0;圣克鲁斯;神经元n = 4)被发现在分散在多个PVN parvocellular细分,包括背侧和腹外侧subnuclei内侧。NPY Y1R-ir也是著名的PVN后magnocellular subnucleus。在不同的实验中,NPY Y1R-ir与vasopressin-ir (1:800;h .获得者)神经元的礼物在这个地区。 While no colocalization of NPY Y1R-ir with CTb-ir (1:1000; AbCam) was observed in the more rostral PVN (1.6 mm from bregma), a significant fraction (1638%) of NPY Y1R neurons in caudal PVN regions (1.8 to 2.1 mm from bregma) co-expressed CTb. In summary, NPY Y1R are expressed in PVN neurons projecting to the RVLM, suggesting that NPY acts directly on these neurons to inhibit sympathetic nerve activity and baroreflex regulation. Supported in part by HL088552.